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It is never too late to mend. - It is the first step that is difficult.
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The author of this page is independent and has no commercial intention in mind ! | Diese Seite in Deutsch. | |||
| Our historical Sample: The same mistake we never did again! |
| What you see here is tha same sample (from the same/identical bottle) in the "identical"
solvent: Ethanol with the quality label - UVASOL.
- It means special medaled produced for spectroscopy -, once from a fresh opened
bottel of Lot A, and once from a fresh opened bottle of Lot B, but all from the identical supplier! And finaly you see the spectrum of the same sample in a mixture of Ethanol/Buffer pH 4.65 (1:1): - (The only spectrum, that can be reproduced again and again in exact the same precision!!!) |
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| Can you imagine, how wrong
your concentration result would be, calculated as usually at Lambdamax. of 654 nm,
if you did the calibration in Alcohol from Lot A and your sample measurement in Alcohol from Lot B ? That's why “old” spectroscopist are telling you, you must measure your reference and your samples not only in the same solvent, they are telling you, you must prepare enough solvent for both measurements and do all time both measurements together ! |
And now my three simple Questions to see what you have just learned:
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| Go back to Spectroscopy |
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Peter Forster:
  
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Software Development & Consulting Peter Forster Neubadstrasse 88 CH-4054 Basle, Switzerland Mail to: peter.forster@p-forster.com |
Die selbe Seite in Deutsch |